Isolation and characterization of Candida kefyr orotidine-5′- phosphate decarboxylase (URA3) gene

Authors

Wai Kei Paul Tsang, Technological and Higher Education Institute of Hong Kong, Vocational Training CouncilFollow
Kin Sing Wong, Chinese University of Hong Kong
Jennifer Ka Man Chu, Chinese University of Hong Kong

Staff Page link

Dr TSANG Wai Kei Paul

Document Type

Journal Article

Publication Date

2010

DOI

10.1002/yea.1732

Abstract

Candida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine-5′-phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C. kefyr URA3 gene spans 801 bp, corresponding to 267 amino acid residues. The functionality of the gene was confirmed by complementation of ura3 auxotrophs of C. albicans and Saccharomyces cerevisiae. Phylogenetic analysis of the deduced amino acid sequence indicated that it shares a high degree of homology with other Candida URA3 homologues. The GenBank Accession No. of the C. kefyr URA3 gene is FJ914763.

Source Publication

Yeast

Volume Number

27

Issue Number

1

ISSN

0749-503X

First Page

53

Last Page

58

Recommended Citation

Tsang, W.,Wong, K.,& Chu, J. (2010). Isolation and characterization of Candida kefyr orotidine-5′- phosphate decarboxylase (URA3) gene. Yeast, 27 (1), 53-58. http://dx.doi.org/10.1002/yea.1732