EHD1 Define endocytic recycling pathway for cell plate formation and root development in arabidopsis

Document Type

Conference Proceeding

Publication Date

2019

Abstract

Recycling of internalized endocytic proteins to the plasma membrane (PM) occurs at the trans-Golgi network (TGN) in plant cells where the secretory and endocytic protein trafficking pathways converge. Little is known about the nature and identity of PM-endocytic recycling vesicles in plants. To characterize the PM-recycling pathway in plants, we have investigated the plant orthologs of Epidermal growth factor receptor substrate 15-Homology Domain (EHD) proteins. Using an inducible RNA interference (RNAi) approach, we showed that knockdown of EHDs in Arabidopsis resulted in reduced mitotic activity and cell number in MZ, and mild dwarfism phenotype. Trafficking of PIN2 and SCAMP1 is not affected, however, there’s a pronounced reduction of KN localisation to developing cell plate, coherent with the defect in cell division. GFP fusion and immunofluorescent labeling identified EHD1 localised on cytosolic structure distinct from the Golgi apparatus, prevacuolar compartment (PVC) and TGN marker, highlighting cell plate during mitosis and accumulate KN during late cytokinesis. Pharmacological studies revealed that Brefeldin A, but neither Concanamycin A nor Wortmannin, inhibits EHD1 PM-recycling. Immuno-EM identified EHD1-labeled compartment as a population of vesicles in close proximity to the TGN, morphologically similar but biochemically distinct from TGN-derived vesicles. Our works have suggested a functional role of EHD1 in endocytic recycling for PM and cell plate.

Source Publication

Japan-Taiwan Plant Biology 2019, March 2019, Nagoya, Japan

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