Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery

Authors

Sai Wang Seto, The Chinese University of Hong Kong
Ho Yeung Lam, The Chinese University of Hong Kong
Wing Sze Lau, The Chinese University of Hong Kong
Alice L.S. Au, The Chinese University of Hong Kong
Tsz Yan Lam, The Chinese University of Hong Kong
Stephen S.C. Chim, The Chinese University of Hong Kong
Sai Ming Ngai, The Chinese University of Hong Kong
Shun Wan Chan, Technological and Higher Education Institute of Hong Kong, Vocational Training CouncilFollow
Tak Yeung Leung, The Chinese University of Hong Kong
John H.K. Yeung, The Chinese University of Hong Kong
Siu Kai Kong, The Chinese University of Hong Kong
George P.H. Leung, University of Hong Kong
Simon M.Y. Lee, University of Macau
Yiu Wa Kwan, The Chinese University of Hong Kong

Staff Page Link

Dr CHAN Shun Wan

Document Type

Journal Article

Publication Date

2009

Keywords

Monoamine oxidase, Preeclampsia, Umbilical artery, 5-hydroxytryptamine

DOI

10.1016/j.ejphar.2008.12.054

Abstract

We investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (Nω-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development.

Source Publication

European Journal of Pharmacology

Volume Number

605

Issue Number

1-3

First Page

129

Last Page

137

Recommended Citation

Seto, S.,Lam, H.,Lau, W.,Au, A.,Lam, T.,Chim, S.,Ngai, S.,Chan, S.,Leung, T.,Yeung, J.,Kong, S.,Leung, G.,Lee, S.,& Kwan, Y. (2009). Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery. European Journal of Pharmacology, 605 (1-3), 129-137. http://dx.doi.org/10.1016/j.ejphar.2008.12.054