Structure and properties of a (1→3)-β-d-glucan from ultrasound-degraded exopolysaccharides of a medicinal fungus

Document Type

Journal Article

Publication Date

2014

Keywords

Cordyceps sinensis, Mycelial fermentation, Exopolysaccharide, Ultrasonic degradation, (1→3)-β-D-glucan, Moisture absorption

DOI

10.1016/j.carbpol.2014.02.040

Abstract

A high molecular weight (MW) exopolysaccaride (EPS) fraction EPS1 was isolated from the fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1 and partially degraded by high-intensity ultrasound (US) into a lower MW fraction EPS1U. EPS1U exhibited a single, symmetric peak on size exclusion chromatography with an average MW of 730kDa by light scattering analysis. It had a much lower intrinsic viscosity (1.7 versus 15.6dL/g) but a much higher solubility in water (77.5 versus 5.1g/L) than EPS1. Based on methylation analysis and NMR spectrometry, the structure of EPS1U was deduced as a (1→3)-β-d-glucan with glucose side chains attached to O-6 position at the branching points. EPS1U showed a high moisture absorption capability comparable to chitosan and urea, suggesting its potential as a moisturizing agent for food and cosmeceutical application. This is the first report on a high MW (1→3)-β-d-glucan isolated from EPS produced by Cordyceps sinensis.

Source Publication

Carbohydrate Polymers

Volume Number

106

First Page

270

Last Page

275

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