Folic acid supplementation modifies b-adrenoceptor-mediated in vitro lipolysis of obese/diabetic (+db/+db) mice
β-adrenoceptor, Lipolysis, Folic acid, Adipocytes, +db/+db mice
The effects of folic acid (5.7 and 71 μg/kg, 4 weeks) consumption on the β-adrenoceptors (β-ARs)–elicited lipolysis in vitro of the abdominal adipocytes of lean/control (+m/+db) and obese/diabetic (+db/+db) mice (female) were investigated. β-AR agonists (salbutamol, a β2-AR agonist; BRL 37344 and CGP 12177, β3-AR agonists; adrenaline, a β-AR agonist)–mediated lipolysis, β2-, and β3-ARs protein expression of the adipose tissues after folic acid consumption were evaluated. Our results demonstrate that a smaller magnitude of the basal (spontaneous) and the β-AR agonists–triggered lipolysis was observed in +db/+db mice, and folic acid supplementation (71 μg/kg) resulted in an improvement of both the baseline and the β-ARs–mediated lipolysis. In controls, a lower β2-and β3-ARs protein expression of the adipose tissues was detected in +db/+db mice, compared to +m/+db mice. In both strains fed with folic acid (71 μg/kg), a reduction of β2-AR protein expression was observed compared to the respective controls. In +db/+db mice, folic acid (5.7 and 71 μg/kg) consumption caused a dose-dependent increase of β3-AR protein expression compared to controls. We demonstrate that lipolysis elicited by β-AR (β2- and β3-ARs) agonists was blunted in +db/+db mice. Folic acid consumption has significant modulatory effects on β-ARs protein expression and lipolysis.
Experimental Biology and Medicine
Lam, T.,Seto, S.,Au, A.,Poon, C.,Li, R.,Lam, H.,Lau, W.,Chan, S.,Ngai, S.,Leung, G.,Lee, S.,Tsui, S.,& Kwan, Y. (2009). Folic acid supplementation modifies b-adrenoceptor-mediated in vitro lipolysis of obese/diabetic (+db/+db) mice. Experimental Biology and Medicine, 234 (9), 1047-1055. http://dx.doi.org/10.3181/0902-RM-44