Pre-column derivatization and gas chromatographic determination of alkaloids in bulbs of Fritillaria

Document Type

Journal Article

Publication Date

1999

Keywords

Derivatization, GC, Alkaloids, Fritillaria alkaloids

DOI

10.1016/S0021-9673(99)00867-5

Abstract

A method of precolumn derivatization GC with FID detection was developed for a simultaneous analysis of five major steroidal alkaloids of Fritillaria species, namely ebeiedine, ebeiedinone, verticine, verticinone and imperialine. Derivatization was carried out by trimethylsilylation of the hydroxyl-containing Fritillaria alkaloids to the corresponding trimethylsilylates with trimethylsilylimidazole. Reaction conditions were optimised and the alkaloids derivatives were characterised by on-line GC–MS. The validated GC method demonstrated a good linearity at the sampling ranges used. This analytical method is simple, convenient and reproducible. The developed assay was successfully applied to the determination of the major pharmacologically active alkaloids in three commonly used antitussive Fritillaria species: F. cirrhosa, F. thunbergii and F. pallidiflora.

Source Publication

Journal of Chromatography A

Volume Number

859

Issue Number

2

First Page

183

Last Page

192

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