Determination of the major isosteroidal alkaloids in bulbs of Fritillaria by high performance liquid chromatography coupled with evaporative light scattering detector

Document Type

Journal Article

Publication Date

2001

Keywords

Fritillaria spp., Isosteroidal alkaloids

DOI

10.1016/S0021-9673(00)01083-9

Abstract

A new direct HPLC analytical method using evaporative light scattering detection coupled with a low-temperature adapter for the simultaneous determination of the major biologically active isosteroidal alkaloids in Bulbus Fritillariae, a commonly used antitussive traditional Chinese medicinal (TCM) herb, has been developed. The simultaneous separation of eight Fritillaria alkaloids was achieved on a reversed-phase C8 column with an isocratic mobile phase system consisting of acetonitrile–methanol–water (66.5:3.5:30, v/v) containing 0.006% triethylamine. This method provides good reproducibility and sensitivity for the quantification of six major isosteroidal alkaloids, namely peimissine, verticine, verticinone, imperialine, isoverticine and ebeiedine in different Fritillaria species with overall intra- and inter-day precision and accuracy of less than 11% and higher than 90%, respectively. The assay was successfully utilized to quantify the major biologically active alkaloids in five Fritillaria species. The results demonstrate that this method is simple, selective, and suitable for the quality control of this commonly used antitussive TCM herb, Bulbus Fritillariae.

Source Publication

Journal of Chromatography A

Volume Number

909

Issue Number

2

First Page

207

Last Page

214

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