"Detection of puerarin and danshensu in traditional Chinese medicinal p" by Xue Na Hao, Shun Wan Chan et al.
 

Detection of puerarin and danshensu in traditional Chinese medicinal preparation containing Pueraria lobata and Salvia miltiorrhiza by high-performance liquid chromatography

Document Type

Journal Article

Publication Date

2007

Keywords

Puerarin, Danshensu, Pueraria lobata, Salvia miltiorrhiza, HPLC

DOI

10.1080/10826070701560942

Abstract

A high performance liquid chromatographic method was developed and applied to the simultaneous determination of puerarin and danshensu in a traditional Chinese medicinal preparation containing Pueraria lobata and Salvia miltiorrhiza as its major herbal constituents. Elution was performed on a C18 column at 27°C with acetonitrile–water (0.5% acetic acid) at different proportions according to a time scheduled programme, and pumped at a flow rate of 1.0 mL/min. Indomethacin was used as an internal standard. High resolution was obtained between the analytes and the internal standard. The intra‐day and inter‐day precisions were better than 1.9% and 2.2%, respectively. The coefficient of determination for each analyte was above 0.997. It is a simple and sensitive analytical method with good accuracy and reproducibility.

Source Publication

Journal of Liquid Chromatography & Related Technologies

Volume Number

30

Issue Number

18

First Page

2779

Last Page

2787

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