Title

An in vitro study of Sanchi (Panax pseudoginseng) for its DNA protective effect

Document Type

Journal Article

Publication Date

2014

Keywords

Acellular comet assay, DNA, Hydrogen peroxide, Lysed cell, Oxidative challenge

DOI

10.1080/09540105.2013.794202

Abstract

Panax pseudoginseng (Shachi) are well known for their antioxidant properties. The aim of this study was to explore Sanchi extracts, potential protection of human cellular DNA from oxidative challenge induced by hydrogen peroxide in vitro. Aqueous and alcoholic extracts of Shachi were incubated with human lymphocytes from 1×10-2% to 1×10-7% (w/v) for 30 mins. Pre-treated cells were then stressed with 50 μM hydrogen peroxide. Washed cells were subjected to the standard and the lysed cell comet assays. Results showed that water extracts of Sanchi at specific concentrations were able to diminish human DNA damage induced by hydrogen peroxide in both standard and lysed cell comet assays. No effect was observed in ethanolic extract. More pronounced protective effect in lysed cell comet assay model implied cell membrane limited the availability of active ingredients of Sanchi to nucleus.

Source Publication

Food and Agricultural Immunology

Volume Number

25

Issue Number

3

ISSN

0954-0105

First Page

332

Last Page

340

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