A study of DNA protective effect of orange juice supplementation

Document Type

Journal Article

Publication Date

2013

Keywords

Antioxidant, Ascorbic acid, Comet assay, Genoprotection, Lymphocyte

DOI

10.1139/apnm-2012-0344

Abstract

The potential acute genoprotective effect of orange juice supplementation was investigated. Six healthy subjects (aged 33 to 60 years ; 3 women and 3 men) were asked to drink 400 mL of commercial orange juice, which contained 100 mg vitamin C and 40.8 g sugar. Venous blood (2 mL) was taken before and 2 h after ingestion (test trial). A week later, the subjects were asked to repeat the trial by drinking 400 mL water with 100 mg vitamin C and 40.8 g glucose (control trial). Lymphocytes isolated from blood samples underwent comet assay on the day of collection. Pre- and postingestion DNA damage scores were measured in both the test and control trials. Results showed that there was a significant decrease in DNA damage induced by hydrogen peroxide after 2 h of supplementation with orange juice, and no change in baseline DNA damage. There was no significant decrease in the DNA damage in lymphocytes in the control trial.

Source Publication

Applied Physiology, Nutrition, and Metabolism

Volume Number

38

Issue Number

5

ISSN

1715-5312

First Page

533

Last Page

536

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