Protective effect of grape seed extracts on human lymphocytes: a preliminary study

Document Type

Journal Article

Publication Date

2013

Keywords

Acellular, Antioxidant, Comet assay, DNA damage, Grape seed extracts

DOI

10.1139/apnm-2012-0296

Abstract

Grape seed extracts (GSEs) possess a broad spectrum of antioxidative properties that protects various cells from free radicals and oxidative stress. In this study, the genoprotective effect of GSE on human lymphocytic DNA was studied using standard and lysed cell comet assays. Lymphocytes from 5 healthy subjects were pretreated with GSE in different concentrations. The standard and lysed cell comet assays were performed on treated, untreated, challenged, and unchallenged cells in parallel. Cells were then subjected to an oxidant challenge induced with 5-min exposures to hydrogen peroxide. In the standard comet assay, GSE significantly diminished hydrogen-peroxide-induced DNA damage in a dose-dependent manner. In the lysed cell assay, however, the antioxidant effect was diminished at a higher GSE concentration. Data indicate that the cell membrane might play a role in limiting cellular access to antioxidants, which directly affects the genoprotective or potential pro-oxidant effect of antioxidants on human DNA. Using both standard and lysed cell comet assays in parallel could be a useful way to elucidate the mechanism of protection or damage by antioxidants.

Source Publication

Applied Physiology, Nutrition, and Metabolism

Volume Number

38

Issue Number

3

ISSN

1715-5312

First Page

275

Last Page

279

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