Luminescent polypyridinerhenium(I) bis-biotin complexes as crosslinkers for avidin

Document Type

Journal Article

Publication Date

2009

Keywords

Crosslinking, Cytotoxicity, Imaging agents, Luminescence, Rhenium, Sensors

DOI

10.1002/ejic.200900518

Abstract

Two novel luminescent polypyridinerhenium(I) bis-biotin complexes [Re(N^N)(CO)3(pyridine)](CF3SO3) [N^N = 4,4'-bis{[2(biotinamido)ethyl]aminocarbonyl}-2,2'-bipyridine, bpyC2B2 (1), 4,4'-bis[(2-{[6-(biotinamido)hex.anoyl]amino}ethyl)aminocarbonyl]-2, 2'-bipyridine, bpyC2C6B2 (2)} and their biotinfree counterpart [N^N = 4,4'-bis(n-butylarninocarbonyl)-2,2'bipyridine, bpyC4 (3)] have been synthesized and characterized, Upon irradiation, all the complexes exhibited triplet metal-to-ligand charge-transfer (3MLCT) emission in fluid solutions at room, temperature and alcohol glass at 77 K. The avidin-binding properties of the bis-biotin complexes 1 and 2 have been studied by 4'-hydroxyazobenzene-2- carboxylic acid (HABA) assays, emission titrations, and dissociation assays. The potential use of the complexes as signal amplifiers for heterogeneous recognition assays has been demonstrated using avidln-coated microspheres and one of the complexes, Additionally, the cytotoxicity of these rhenium(I) complexes towards the human cervix epithelioid carcinoma (HeLa) cell line has been evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5diphenyltetrazolium bromide (MTT) assays, Furthermore, the cellular uptake of the complexes has been examined by laser-scanning confocal microscopy.

Source Publication

European Journal of Inorganic Chemistry

Volume Number

2009

Issue Number

28

ISSN

1434-1948

First Page

4265

Last Page

4273

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