Single‐cell gel electrophoresis: a tool for investigation of DNA protection or damage mediated by dietary antioxidants

Authors

Yim Tong Savio Szeto, Hong Kong Institute of Vocational Education (Shatin), Vocational Training CouncilFollow

Document Type

Journal Article

Publication Date

2007

Keywords

Antioxidant, Comet assay, Dietary, DNA, Pro‐oxidant

DOI

10.1002/jsfa.3039

Abstract

One method of assessing DNA damage is the comet assay, which was developed in 1988. The comet assay enables the detection of DNA strand breaks in individual cells. This test has also been used to study the in vitro and in vivo genotoxic or genoprotective effects of certain agents such as dietary antioxidants. This paper aims to consolidate the antioxidant and pro‐oxidant effects of a series of dietary agents which have been evaluated by comet assay.

Source Publication

Journal of the Science of Food and Agriculture

Volume Number

87

Issue Number

13

ISSN

0022-5142

First Page

2359

Last Page

2381

Recommended Citation

Szeto, Y. (2007). Single‐cell gel electrophoresis: a tool for investigation of DNA protection or damage mediated by dietary antioxidants. Journal of the Science of Food and Agriculture, 87 (13), 2359-2381. http://dx.doi.org/10.1002/jsfa.3039