Title
Isolation and characterization of Candida kefyr orotidine-5′- phosphate decarboxylase (URA3) gene
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Document Type
Journal Article
Publication Date
2010
DOI
10.1002/yea.1732
Abstract
Candida kefyr is a common yeast species that can be found in fermented milk and cheeses. As a first step to developing a gene transfer system for C. kefyr, the orotidine-5′-phosphate decarboxylase (URA3) gene was cloned, using degenerate PCR and genome walking. The uninterrupted open reading frame of the C. kefyr URA3 gene spans 801 bp, corresponding to 267 amino acid residues. The functionality of the gene was confirmed by complementation of ura3 auxotrophs of C. albicans and Saccharomyces cerevisiae. Phylogenetic analysis of the deduced amino acid sequence indicated that it shares a high degree of homology with other Candida URA3 homologues. The GenBank Accession No. of the C. kefyr URA3 gene is FJ914763.
Source Publication
Yeast
Volume Number
27
Issue Number
1
ISSN
0749-503X
First Page
53
Last Page
58
Recommended Citation
Tsang, W.,Wong, K.,& Chu, J. (2010). Isolation and characterization of Candida kefyr orotidine-5′- phosphate decarboxylase (URA3) gene. Yeast, 27 (1), 53-58. http://dx.doi.org/10.1002/yea.1732