Endocytic recycling vesicles function in root development

Document Type

Conference Proceeding

Publication Date



Recycling of internalized endocytic proteins to the plasma membrane (PM) occurs at the trans-Golgi network (TGN) in plant cells where the secretory and endocytic protein trafficking pathways converge. Little is known about the nature and identity of PM-recycling vesicles in plants. A family of proteins containing the Epidermal growth factor receptor substrate 15-Homology Domain (EHD) are markers of recycling, and EHD bearing tubules function in slow-recycling of internalized proteins from sorting endosome to the PM in animal cells. To define the PM-recycling pathway in plants, we cloned the Arabidopsis homolog of mammalian EHD1 and generated a green fluorescent protein (GFP)-fusion and expressed it in tobacco BY-2 cells and Arabidopsis plants. Cytosolic punctate structures labeled by GFPEHD1 fusion or endogenous EHD1 are distinct from the Golgi apparatus and prevacuolar compartment (PVC), but partially colocalize with a TGN marker. Immunogold electron microscopy of high-pressure frozen/freeze-substituted samples identified the EHD1-labeled compartment as a population of vesicles in close proximity to the TGN. These are morphologically similar but biochemically distinct to TGN-derived vesicles. Using an inducible RNA interference (RNAi) approach, we showed that EHD1 knockdown by RNAi could hamper root development and cell plate formation in Arabidopsis.

Source Publication

Plant Biology 2017, June 2017, Hawaii, USA

This document is currently not available here.