Mouthrinse effectiveness against Candida glabrata

Document Type

Conference Proceeding

Publication Date



Objectives: To investigate the effectiveness of polyaminopropylbiguanide (ProntoLind), sodium chlorite (TheraBreath), povidone iodine (Betadine), Hamamelis virginiana extract (Systema), octenidine hydrochloride (Octenidol), chlorhexidine digluconate (Corsodyl), and essential oil mouthrinses (Listerine) against C. glabrata planktonic cells and biofilms.

Methods: Standardized inocula of C. glabrata ATCC 90030 were prepared and minimum inhibitory concentrations (MICs) of the mouthrinses were determined in accordance with Clinical and Laboratory Standards Institute (CLSI) Guidelines for the broth microdilution assay. Minimum fungicidal concentrations (MFCs) were determined by culturing optically clear wells. Mouthrinse efficacy against pre-formed biofilms was evaluated after one minute, and after five minutes of exposure.

Results: Octenidine hydrochloride and polyaminopropylbiguanide exhibited the highest MICs (1:2048 dilution), while the latter agent and chlorhexidine demonstrated fungicidal activity at 1:512 dilution. The essential oil exhibited significantly greater anti-biofilm activity (p≤0.002) than the other mouthrinses at an exposure time of one minute, with a 52% reduction in viable counts. Chlorhexidine reduced viable counts by 37%, and was significantly more effective than polyaminopropylbiguanide (p=0.001), sodium chlorite (p>0.001), povidone iodine (p=0.001), Hamamelis virginiana extract (p

Conclusions: The essential oil mouthrinse exhibited the greatest anti-biofilm activity at both one and five minute exposure times. The standard rinsing time of one minute recommended by most commercial mouthrinse manufacturers may be insufficient for the reduction of C. glabrata.

Source Publication

The 29th Annual Scientific Meeting International Association for Dental Research, Bali, Indonesia

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