An in vitro study of Sanchi (Panax pseudoginseng) for its DNA protective effect
Acellular comet assay, DNA, Hydrogen peroxide, Lysed cell, Oxidative challenge
Panax pseudoginseng (Shachi) are well known for their antioxidant properties. The aim of this study was to explore Sanchi extracts, potential protection of human cellular DNA from oxidative challenge induced by hydrogen peroxide in vitro. Aqueous and alcoholic extracts of Shachi were incubated with human lymphocytes from 1×10-2% to 1×10-7% (w/v) for 30 mins. Pre-treated cells were then stressed with 50 μM hydrogen peroxide. Washed cells were subjected to the standard and the lysed cell comet assays. Results showed that water extracts of Sanchi at specific concentrations were able to diminish human DNA damage induced by hydrogen peroxide in both standard and lysed cell comet assays. No effect was observed in ethanolic extract. More pronounced protective effect in lysed cell comet assay model implied cell membrane limited the availability of active ingredients of Sanchi to nucleus.
Food and Agricultural Immunology
Szeto, Y.,Tse, R.,Benzie, I.,Kalle, W.,& Pak, S. (2014). An in vitro study of Sanchi (Panax pseudoginseng) for its DNA protective effect. Food and Agricultural Immunology, 25 (3), 332-340. http://dx.doi.org/10.1080/09540105.2013.794202